Encapsulated Phytase Produced by Recombinant Yarrowia lipolytica Exhibits High Efficiency on Broiler Chickens in Low Dosage

Phytases are the largest group of feed enzymes increasing accessibility organic phosphorus for animals. Feed phytases usually sold as dried powder secreting producers, mainly micellar fungi. We proposed a new technology producing phytase from Obesumbacterium proteus (OPP) in yeast Yarrowia lipolytica cytosolic protein (encapsulated OPP), where capsule (yeast cell) protects enzyme unfavourable factors (acid medium and active proteolysis stomachs) releases it along with substrate duodenum only. Here we report results testing encapsulated OPP on model broiler chicken comparison to conventional Aspergillus ficuum. The at dosage 30 FYT/kg provided maximum body weight end experiment equal or somewhat higher than control group, available deficit was complemented mineral supply. In contrast, soluble 100 1000 not able compensate diet, although chemical analysis demonstrated much diet non-accessible form. decreased residual Pi faeces by 2.1 times when added whereas negligibly affected this parameter regardless dosage.